Identify presence of contaminants

Whenever there is risk of your product containing proteins that are structurally different, HOS comparisons can confirm structural purity.

Quality Control Case Study Graphs 1

HOS Comparisons for spiking experiments

In this example, an antibody-based therapeutic was spiked with different amounts of bovine serum albumin (BSA), representative for contaminants that are structurally different, and with different amounts of an aggregated fraction of the same product (Agg.), representative for contaminants that are structurally highly similar.

The highest contents of BSA and aggregated product resulted in far-UV CD spectra that are easily visually distinguished from the spectrum of the pure product.

Quality Control Case Study Graphs 2

Identify small amounts of contaminant

Smaller amounts of contaminants were only distinguishable by means of statistical analysis. Similarity between samples was assessed by calculating Weighted Spectral Difference values.

BSA contents of all amounts tested could be discerned from the pure product, and contents of aggregated product down to 20% were unambiguously identified.

Find out more!

Have a look at the system that was used in this example.