This study examined binding of the mRNA 5' cap to the translation initiation factor eIF4E.
Comprehensive near-UV CD data revealed differences between protein homologues from human (H. sapiens), fly (D. melanogaster), and yeast (S. cerevisiae).
Homologue-dependent spectral contributions from tryptophan residues Trp56 and Trp102 that are located in the cap binding site reflected different orientations upon binding.
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