Dual Fluorescence Detection
The accessory comprises an additional fluorescence detector
to enable simultaneous fluorescence detection at two emission
wavelengths. Both detectors are mounted directly onto the cell
block. Alternatively, one of the fluorescence detectors can
be mounted on a second (emission) monochromator allowing for
the emission wavelength to be selected directly from the Pro-Data
control software.
This dual fluorescence configuration is particularly useful in applications using FRET (Förster resonance energy transfer), where a donor fluorophore transfers energy to an acceptor fluorophore, producing a fluorescence emission signal at a longer wavelength. The efficiency of this nonradiative transfer is exceptionally sensitive to the distance between these two fluorophores, giving researchers insight into protein-protein interactions, enzyme-substrate binding, and membrane processes.
Features:
- Simple mounting to the SHU cell block/emission mono
- No need for hardware reconfiguration while switching back to single-channel mode
- A variety of available cut-off and interference filters are available
- Possibility to use the option in tandem with the scanning monochromator for emission spectra recording