Chirascan V100

THE HIGH-PERFORMANCE CIRCULAR DICHROISM INSTRUMENT

Suitable for studying chiral molecules of all types and sizes, the sensitivity and accuracy of a Chirascan V100 circular dichroism spectrometer ensure that researchers get the most from every CD analysis, generating high-quality data that contribute to a deeper understanding of biomolecular characteristics, mechanisms, and interactions.

More than α-helix and β-sheet—determine structural and thermodynamic properties

Achieve highest sensitivity and accuracy
Gain insight and detect changes in secondary and tertiary structure
Determine response to thermal or chemical change
Study folding and unfolding mechanisms
Expand capabilities with dedicated Chirascan accessories

Performance

Increased sensitivity when sample is limited

Avalanche photodiode detector enhances sensitivity
Higher signal:noise ratio than conventional photomultiplier tube detectors
Accurate normalization from simultaneous measurement of absorbance and CD

Since their introduction in 2005, Chirascan™ systems have continued to feature in thousands of peer-reviewed publications covering a wide range of research areas. The Chirascan V100 now offers the increased sensitivity and accuracy preferred for CD analysis of biomolecules.

Calibration with confidence

Accurate CD values across entire wavelength range
Overcome challenges of chemical calibration
Optics-based, multiwavelength calibration

Conventional chemical calibration methods require considerable skill in preparation. Standards, such as camphor-10-sulfonic acid (CSA), are unstable, photolabile, and hygroscopic. In addition, single-wavelength calibration (290.5 nm) assumes the same linear response at all wavelengths.

The optics-based, multiwavelength calibration method used for Chirascan V100 and Chirascan Q100 systems overcomes these challenges. The correct calibration is applied to every wavelength to yield accurate CD values.

Publications

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Features and accessories

Chirascan systems are supplied with the features and accessories required for acquisition of high quality data from ‘day one’.

Molecular sieve, activated charcoal filter

Removes common gas impurities

Active nitrogen management system

Regulates purge gas consumption
Software-controlled

Air-cooled xenon lamp

Software-controlled
Up-time recorded

Monochromator

Produces horizontally, linearly polarized monochromatic light
Dual polarizing prisms maximize light throughput

Photoelastic modulator

Converts horizontally polarized light to circularly polarized light. Alternates between left- and right-handed circular polarized light

Temperature-controlled sample chamber

Consistent analytical conditions
Continuous temperature ramps
Temperature measured directly in sample

Water circulator

Dissipates heat from sample chamber and sample storage Peltiers

Avalanche photodiode detector

Highest sensitivity (high signal: noise)

Optics-based, multiwavelength calibration

For CD accuracy

Cuvettes and holders

Selected for far- and near-UV CD analysis of biomolecules (single sample mode)

Customer Experiences

Dr Dan Pantos

Dr Dan Pantos, a Senior Lecturer and Head of Organic Chemistry at the University of Bath, utilises the Chirascan CD instrument from Applied Photophysics for various research projects. His primary research involves studying the interactions between chiral small molecules and quadruplex DNA, with the goal of developing anti-cancer treatments.

Dr Tonya Zeczycki

Dr Tonya Zeczycki, an associate professor at the Brody School of Medicine at ECU, studies Alzheimer's, Parkinson's, and other neurodegenerative diseases in her lab. She focuses on protein interactions, misfolding, and aggregation.

Dr Nick Larson

Dr. Nick Larson, a seasoned expert in protein and oligonucleotide therapeutics, leverages the Chirascan CD spectrometer in his work on a wide range of therapeutics, from protein-based drugs to advanced gene-silencing molecules.

User Cases

Applied Photophysics is a company with a prestigious legacy and has been supporting scientists for over 50 years.

Peptide Therapeutics

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Protein Therapeutics

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